Open Access Peer-reviewed

Multidrug Resistance-1 Gene Polymorphisms in Steroid Resistance Nephrotic Syndrome with Different Pathology

Doaa M Youssef1,, Amal S El-Shal2

1Department of Pediatrics, Zagazig University, Zagazig, Egypt

2Department of Medical Biochemistry, Zagazig University, Zagazig, Egypt

American Journal of Medicine Studies. 2013, 1(1), 1-3. DOI: 10.12691/ajms-1-1-1
Published online: August 25, 2017

Abstract

MDR-1 expression in lymphocytes has been reported to be negatively correlated with the response to prednisone in children with nephrotic syndrome (NS). The aim of this study was to evaluate if MDR-1 gene polymorphisms are different according to pathological type of steroid resistant NS or not. We studied 15 cases with SRNS; 11 cases with focal segmental glomerulosclerosis (FSGS) and 4 cases with mesangioproliferative glomerulonepheritis. All cases were tested for 1 (MDR-1) genetic polymorphisms [C1236T, G2677T (A) and C3435T] polymorphisms; this was done by PCR-based restriction fragment length polymorphism (RFLP). By comparing these polymorphisms between FSGS cases versus mesangioproliferative glomerulonepheritis cases we didn’t find any significant difference in either genotypes or alleles. Thus we concluded that there is no relation between pathological type of SRNS and MDR-1 gene polymorphism in the three tested sites [C1236T, G2677T/A, C3435T].

Keywords:

MDR-1 gene, FSGS, steroid resistant, nephrotic syndrome, children
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