Open Access Peer-reviewed

Multidrug Resistance-1 Gene Polymorphisms in Steroid Resistance Nephrotic Syndrome with Different Pathology

Doaa M Youssef1,, Amal S El-Shal2

1Department of Pediatrics, Zagazig University, Zagazig, Egypt

2Department of Medical Biochemistry, Zagazig University, Zagazig, Egypt

American Journal of Medicine Studies. 2013, 1(1), 1-3. DOI: 10.12691/ajms-1-1-1
Published online: August 25, 2017


MDR-1 expression in lymphocytes has been reported to be negatively correlated with the response to prednisone in children with nephrotic syndrome (NS). The aim of this study was to evaluate if MDR-1 gene polymorphisms are different according to pathological type of steroid resistant NS or not. We studied 15 cases with SRNS; 11 cases with focal segmental glomerulosclerosis (FSGS) and 4 cases with mesangioproliferative glomerulonepheritis. All cases were tested for 1 (MDR-1) genetic polymorphisms [C1236T, G2677T (A) and C3435T] polymorphisms; this was done by PCR-based restriction fragment length polymorphism (RFLP). By comparing these polymorphisms between FSGS cases versus mesangioproliferative glomerulonepheritis cases we didn’t find any significant difference in either genotypes or alleles. Thus we concluded that there is no relation between pathological type of SRNS and MDR-1 gene polymorphism in the three tested sites [C1236T, G2677T/A, C3435T].


MDR-1 gene, FSGS, steroid resistant, nephrotic syndrome, children
[1]  Ahmad H, Tejani A. Predictive value of repeat renal biopsies in children with nephrotic syndrome. Nephron 2000; 84: 342-346.View Article  PubMed
[2]  Daskalakis N, Winn MP. Human genome and diseases. Cell Mol Life Sci 2006; 63: 2506-11.View Article  PubMed
[3]  Klimecki WT, Futscher BW, Grogan TM et al. P-glycoprotein expression and function in circulating blood cells from normal volunteers. Blood 1994; 83: 2451-2458. PubMed
[4]  Cascorbi I, Gerloff T, Johne A, Meisel C, Hoffmeyer S, Schwab M, Schaeffeler E, Eichelbaum M, Brinkmann U, Roots I. Frequency of single nucleotide polymorphisms in the P-glycoprotein drug transporter MDRl gene in white subjects. Clin Phurmacol The 2001, 69: 169-174.View Article  PubMed
[5]  Wasilewska A, Zalewski G, Chyczewski L et al. MDR-1 gene polymorphisms and clinical course of steroid-responsive nephritic syndrome in children. Pediatr Nephrol 2007; 22: 44-51.View Article  PubMed
[6]  Woodahl EL, Ho RJ. The role of MDR1 genetic polymorphisms in interindividual variability in P-glycoprotein expression and function. Curr Drug Metab 2004; 5: 11-19.View Article  PubMed
[7]  Youssef DM, Elbhidy RE, Abdelhalim HS, Amr Gh.. Soluble Interleukine-2 Recptor and MDR1 Gene Expression Levels as Inflammatory Biomarkers for Prediction of Steroid Response in Children With Nephrotic Syndrome. Iranian Journal of Kidney Diseases.2011; 5, 154-161. PubMed
[8]  Jafar T, Prasad N, Agarwal V, Mahdi A, Gupta A, Sharma RK, Negi MP, Agrawal S. MDR-1 gene polymorphisms in steroid-responsive versus steroid-resistant nephrotic syndrome in children. Nephrol Dial Transplant. 2011 Dec; 26(12):3968-74.View Article  PubMed