Figure 10. Amplification plot showing ten fold serial dilutions of CHIKV in-vitro transcribed RNA (10⁸- 10¹) detected by Real Time PCR. PCR cycles (X axis) were plotted against fluorescence intensity (Y axis). The threshold cycle (Ct) is inversely proportional to starting amount of target material. Detection limit of Real Time RT-PCR for CHIKV: 10¹copies/ml. B). Standard curve of showing ten fold serial dilutions of CHIKV in-vitro transcribed RNA detected by Real Time RT-PCR

From

A Comprehensive Prospective Study to Understand Chikungunya Infection in Delhi Region during 2010-2011

Singh Pradeep Kumar, Dar Lalit, Broor Shobha

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