Figure 3. Agarose gel (1 %) detection of the mutation in the gyrA gene and parC gene by HinfI restriction fragment length polymorphism of PCR products. Lane 1 for each gene: non-digested PCR product indicating mutation in the QRDR of this gene. Lane 2 for each gene: digested PCR product indicating no mutation in the QRDR of this gene. The rightest lane: DNA molecular weight marker


PCR-RFLP-Based Detection of Mutations in the Chromosomal Fluoroquinolone Targets gyrA and parC Genes of Acinetobacter baumanii Clinical Isolates from a Tertiary Hospital in Cairo, Egypt

Mahmoud M. Tawfick, Mervat I. El-Borhamy

American Journal of Microbiological Research. 2017, 5(2), 37-43 doi:10.12691/ajmr-5-2-3