Figure 2. A. PCR amplification of TEM gene of the E. coli isolate on 2% agarose gel electrophoresis. Lane 1 DNA ladder: MW 100-1500bp. Lane 2 control negative. Lane, 3 control positive Lane 4 showing typical band size of (971bp) corresponding to the molecular size of TEM gene. B. Mutant TEM gene sequence chromatogram, shown by FinchTV software. C. BioEdit multiple sequence alignment. The inserted and deleted G in Sudan isolate illustrated by the arrows

From

Molecular Characterization and in Silico Analysis of a Novel Mutation in TEM-1 Beta-Lactamase Gene among Pathogenic E. coli infecting a Sudanese Patient

Hisham N Altayb, Nagwa M El Amin, Maowia M. Mukhtar, Mohamed Ahmed Salih, Mohamed A M Siddig

American Journal of Microbiological Research. 2014, 2(6), 217-223 doi:10.12691/ajmr-2-6-8