Isolation and identification of dye decolorizing bacterial isolate from textile dye effluent was carried out. The isolates of Bacillus spp. ETL-79 were isolated from the textile effluent samples collected from textile industries of Ankleshwar, Gujarat, India. Different parameters were used for optimizing. Conditions for maximum decolorization depend on the bacterial isolate. The results showed that the temperature (40°C), pH (8.00), Biological Oxygen Demand (220 mgl–1), Chemical Oxygen Demand (700 mgl–1), Total Suspended Solids (2800 mgl–1), Total Dissolved Solids (7500 mgl–1) and color over the prescribed fresh water limits. A potential bacterial strain was isolated and selected from the textile effluent on the basis of rapid azo dye Crystal violet (100mgl–1) decolorization and later identified as belonging to genus Bacillus based on Phenotypic characterization Effects of physicochemical parameters (pH, Temperature, etc.) on the Crystal violet decolorization by the Bacillus spp ETL-79 were studied. Decolorization was effective at pH 8, 35°C with starch and peptone as carbon and nitrogen sources and in static conditions. This decolorization potential increased the applicability of this microorganism for the dye removal.
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