Open Access Peer-reviewed

Optimization of Environmental Parameters on Decolorization of Remazol Black B Using Mixed Culture

Maulin P Shah1,, Patel KA1, Nair SS1, Darji AM1, Shaktisinh J Maharaul2

1Industrial Waste Water Research Laboratory, Division of Applied & Environmental Microbiology Lab, Enviro Technology Limited, Gujarat, India

2Laboratory of Environmental Bioremediation, Narmada Clean Tech Limited (FETP), Guajrat, India

American Journal of Microbiological Research. 2013, 1(3), 53-56. DOI: 10.12691/ajmr-1-3-4
Published online: August 25, 2017


In the present study, an attempt was made to examine the potential of mixed culture (contains Pseudomonas putida, Psueomonas stutzeri, Pseudomonas aeruginosa, Pseudomonas mendocina) for decolorization of Remazol Black B dye in batch reactors. The effect of pH, temperature, inoculum, initial concentration of dye and initial concentration of glucose was studied with an aim to determine the optimal conditions required for maximum decolorization and degradation. The culture exhibited maximum decolorization ability at pH between 7-8 and at 30°C. A 10% (v/v) inoculum and 1% (w/v) glucose concentration were found to be the optimum for decolorization. A maximum of 98% decolorization was observed at 25 ppm initial concentration of dye after 18 hours of incubation period. At higher dye concentration of 300 ppm, the removal in colour was found to be 75% in 48 hours of incubation period. The results show that the enriched mixed culture from activated sludge has an excellent potential in removal of Remazol Black B dye from wastewater under aerobic conditions.


Remazol Black B, pH, temperature, decolorization
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