Open Access Peer-reviewed

Protease Production by Fusarium oxysporum in Solid- State Fermentation Using Rice Bran

Syed Sajeed Ali1,, N.N. Vidhale1

1Department of Microbiology, Shri Shivaji Science College Amravati, Amravati, India

American Journal of Microbiological Research. 2013, 1(3), 45-47. DOI: 10.12691/ajmr-1-3-2
Published online: August 25, 2017

Abstract

Agroindustrial wastes constitute valuable source for microbial cultivation and enzyme production. An attempt was made to study protease production by Fusarium oxysporum on agroindustrial waste rice bran under solid-state fermentation. Rice bran obtained from rice mill has proved to be efficient substrate for enzyme production by F. oxysporum. To study the maximum enzyme production the process parameters of fermentation where optimized. Maximum protease production i.e. (70.5U/g) was obtained with an initial moisture content of 50% (w/w) on incubation period of 72h at 35°C temperature with initial pH of substrate 7.0. The above data revealed that agroindustrial waste rice bran and optimized condition of solid-state fermentation can be successfully employed for protease production by Fusarium oxysporum.

Keywords:

agroindustrial waste, protease, Fusarium oxysporum, solid -state fermentation
[1]  Agrawal, D., Partidar, P., Banerjee, T., and Patil, S., “Alkaline protease production by a soil isolate of Beauveria feline under SSF condition: parameter optimization and application to soy protein hydrolysis,” Process Biochem, 40, 1131-1136. 2005.View Article
 
[2]  Alagarsamy, S., Paul, D., Chandran, S., George, S., Carlos, R., “Rice bran as a substrate for proteolytic enzyme production,” Brazilian Archives of Biology and technology, 49, 843-851. 2006.View Article
 
[3]  Alexopoulos, C.J., “Introductory Mycology” IInd edition. John Wiley and sons, New York. 1962.
 
[4]  Anonymous, “International Rules of Seed Testing,” Proc. Int. Seed Test. Assoc., 4, 3-49. 1976.
 
[5]  Chisti, Y., “Solid substrate fermentations, enzyme production, food enrichment. In Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation,” Vol 5, ed. by Flickinger MC and Drew SW. Wiley, New York, 2446-2462. 1999.
 
[6]  Ellaiah, P., Srinivasulu, B., Adinarayana, K., “A review on microbial alkaline proteases,” J. Sci Ind Res, 61, 690-704. 2002.
 
[7]  Gerlach, W., Nirenberg, H., “The genus Fusarium: a pictorial atlas.” Mitteilungen aus der Biologischen Bundesanstalt für Land-und Forstwirtschaft, Berlin-Dahlem, Germany, 1982.
 
[8]  Gupta, R., Beg, Q.K., Lorenz, P., “Bacterial Alkaline Protease: Molecular approaches and industrial application,” Appl Microbial Biotechnol, 59, 15-32. 2002.View Article  PubMed
 
[9]  Jarun, C., Sinsupha, C., Yusuf, C., and Penjit, S., “Protease Production by Aspergillus oryzae in solid-state fermentation using agroindustrial substrate,” J. Chem Tech Biotechnology, 83, 1012-1018. 2008.View Article
 
[10]  Kumar, A., Sachdev, A., Balasubramanyam, S.D., Saxena, A.K., and Lata., “Optimization of conditions for production of neutral and alkaline protease from species of Bacillus and Pseudomonas,” Ind. J. Microbiol. 42, 233-236. 2002.
 
[11]  Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., “Protein measurement with the Folin phenol reagent,” J. Biol Che, 193, 265-275. 1951. PubMed
 
[12]  Negi, S. and Benerjee, R., “Optimization of amylase and protease production from Aspergillus awamori in single bioreactor through EVOP factorial design technique,” Food Technol Biotechnol. 44, 257-261. 2006.
 
[13]  Pandey, A., Sevalkumar, P., Soccol, C.R. and Nigam, P., “Solid state fermentation for the production of industrial enzymes,” Curr Sci, 77, 149-162. 1999.
 
[14]  Paranthaman, R., Alagusundaram, K. and Indhumathi, J., “Production of protease from rice mill wastes by Aspergillus niger in solid state fermentation,” World J. Agri Sci, 5, 308-312. 2009.
 
[15]  Sandhya, C., Sumantha, A., Szakacs, G. and Pandey, A., “Comparative evolution of Neutral proteases production by Aspergillus oryzae in submerged and slid state-fermentation,” Process Biochem., 40, 2689-2694. 2005.View Article
 
[16]  Wang, R., Law, R.C.S. and Webb, C., “Protease Production and Condition by Aspergillus oryzae in Flour fermentation,” Process Biochem, 40, 217-227. 2005.View Article
 
[17]  Yeoman, K.H., and Edwards, C., “Protease production by Streptomyces thermovulgaris grown on rapemeal-derived media,” J. Appl Bacteriol, 77, 264-270. 1994.View Article  PubMed