Figure 2. Effects of TNC knockdown on breast cancer cell invasion and proliferation. (A) 2D invasion assay for MDA-MB-231 cell line transfected with specific isoforms siRNA compared to untreated cells and cells treated with scrambled siRNA, using 5% FCS DMEM as the chemotactic source. Fluorescence values were measured every 2 hours for 48 hours. Graphs represent the difference in fluorescence from time 0. Error bars represents standard error of the mean (6 replicates). (C) Analysis of pHH-3 staining on MDA-MB-231 transfected with total TNC and specific isoforms siRNAs. The graph was generated based on the ImmunoRatio image analysis application, which calculate the percentage of pHH-3 positive staining within five fields. This experiment was performed in triplicate, and the graph shows the mean of five fields (approximately 200 cells per field). (D) Immunocytochemistry staining of pHH-3 expression. MDA-MB-231 cells were transfected in triplicate with total TNC and specific isoforms siRNAs (Magnification is x40). All statistical tests were Two-way ANOVA and Posthoc Tukey test between siRNA treatments and time in cells transfected with siRNAs compared to the cells transfected with scrambled siRNA

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Tenascin-C (TNC) Promotes Breast Cancer Cell Invasion and Proliferation: Functional Effects of TNC Knockdown in Highly Invasive Breast Cancer Cell Lines

Ali S Alharth, Musaad A Alsuliman, Abo Baker I Alshomrani, Waleed A Alyami

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