Figure 2. Electrophoretic typical gel analysis for the effect of LPS/SB203580 on IκB-α cytosolic phosphorylation. (A) Cell cultures were pretreated with ascending concentrations of SB203580 for 1h, followed by incubation with LPS for 24h. The phosphorylated (pIκB-α) and non-phosphorylated forms of IκB-α were subsequently determined. Semi-quantitative loading per lane is verified by the consistent expression of the constitutive form of β-actin (B) Histogram analysis of the relative levels of pIκB-α and IκB-α with selective MAPKp38 inhibition. The number of experimental observations is n = 3 – 5, for separate and independently prepared cell cultures of alveolar epithelial cells with or without pre-treatments with LPS/SB203580


The Irreversible Inhibition of the MAPKp38 Pathway Downregulates LPS-augmented Release of Interleukin-Related Inflammatory Cytokines (IL-1β, IL-6): Immune Surveillance Unraveling IκB-α/NF-κB Phosphorylation State-independent Mechanism in vitro

John J Haddad

American Journal of Medical and Biological Research. 2015, 3(5), 133-138 doi:10.12691/ajmbr-3-5-3