Effect of Alcohol Consumption on Oxidative Stress Markers and its Role in the Pathogenesis and Progression of Liver Cirrhosis
1Department of Biochemistry, Government Medical college, Nagpur
2Department of Biochemistry, Chalmeda Anandarao Inastitute of Medical Sciences, Karimnagar
3Department of Biochemistry, Vaydehi Institute of Medical Sciences and research center, Bengaluru
4Department of Microbiology, Prathima Institute of Medical Sciences, Karimnagar
Oxidative stress has been increasingly implicated in the pathogenesis and progression of liver cirrhosis. Chronic ethanol consumption induces an oxidative stress resulting in increased ferritin levels and thereby iron over load. The study was aimed at evaluating the relation between alcohol consumption and erythrocyte superoxide dismutase (SOD), Glutathione peroxidase (GPx), Malondialdehyde (MDA) activities in liver cirrhosis patients. The study included two groups based on alcohol consumption; subjects taking low alcohol content (Less than 150 g/day.), those consuming high alcohol content (More than 150 g/day), diagnosed as suffering from liver cirrhosis and control group who do not take alcohol and are not suffering from liver cirrhosis. All cirrhotic patients on high alcohol intake in the study group had lower serum SOD (P < 0.0001), GPx (0.0001) and significantly higher MDA levels (P<0.001) than those with subjects taking low alcohol and control group. These results suggest that the decrease in erythrocyte SOD, GPx and increase in MDA levels are related to the alcohol consumption and that may be associated with pathogenesis and progression of liver disease.
Keywords: liver cirrhosis, alcohol consumption, malondialdehyde, superoxide dismutase, Glutathione peroxidase
American Journal of Medical and Biological Research, 2013 1 (4),
Received September 02, 2013; Revised October 10, 2013; Accepted October 11, 2013Copyright © 2014 Science and Education Publishing. All Rights Reserved.
Cite this article:
- Deshpande, Neelesh, et al. "Effect of Alcohol Consumption on Oxidative Stress Markers and its Role in the Pathogenesis and Progression of Liver Cirrhosis." American Journal of Medical and Biological Research 1.4 (2013): 99-102.
- Deshpande, N. , Kandi, S. , Kumar, P. V. B. , Ramana, K. V. , & Muddeshwar, M. (2013). Effect of Alcohol Consumption on Oxidative Stress Markers and its Role in the Pathogenesis and Progression of Liver Cirrhosis. American Journal of Medical and Biological Research, 1(4), 99-102.
- Deshpande, Neelesh, Sabitha Kandi, P Venkata Bharath Kumar, K V Ramana, and Manohar Muddeshwar. "Effect of Alcohol Consumption on Oxidative Stress Markers and its Role in the Pathogenesis and Progression of Liver Cirrhosis." American Journal of Medical and Biological Research 1, no. 4 (2013): 99-102.
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Oxidative stress is defined as an imbalance between pro-oxidant and antioxidant mechanisms in human where the balance is being tilted in favor of the former . Ethanol plays a significant role in the pathogenesis and progression of liver disease. Though liver breaks down the majority of ingested ethanol, it cannot metabolize beyond a specific threshold level . Oxidative stress plays an important role both in non-infectious and infectious conditions such as anemia, hepatocellular carcinomas and infectious diseases [3, 4, 5, 6]. The available literature suggests that intermediates of oxygen reduction may be associated with the development and progression of liver diseases .
Increased production and accumulation of free radical in liver plays an important role in the pathogenesis and progression of liver disease [8, 9, 10, 11]. Previous studies have confirmed that a lipid peroxidation product, malondialdehyde(MDA), stimulates directly or indirectly the lipocytes (Ito cells) resulting in liver fibrosis [12, 13, 14]. Existing literature has established the fact that alcohol consumption induces lipid peroxidation and oxidative stress . Peroxidative lipid decomposition is suggested as a mechanism of hepatocellular injury in parenchymal iron overload . These findings point out the importance of iron and MDA in the progression of liver cirrhosis (LC) . Moreover, little is known about the role of Superoxide dismutase (SOD) in LC. The aim of this study is to evaluate activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA), in chronic alcoholic and cirrhotic patients (low and high alcohol consumption) and to determine the effect of increasing alcohol intake on the pathogenesis and progression of liver disease.
2. Patients and Methods
The study included fifty patients with alcoholic liver cirrhosis and the study subjects are divided in to two groups based on alcohol consumption. Subjects included were grouped as those consuming low alcohol content (less than 150 g/day.) (ALC1), consuming high alcohol content (more than 150 g/day) (ALC2) and control group who do not consume alcohol and are not suffering from liver cirrhosis. 28 patients were included in low alcohol consuming group (ALC1) with a mean age of 42 ± 8 years and 22 patients were included in high alcohol consuming group (ALC2) with a mean age of 42 ± 8 years. 90 individuals with no history of alcohol consumption and without liver cirrhosis are recruited as controls in the study. Patients were obtained from gastroenterology ward of Government medical college and superspeciality teaching hospital with proven history of liver cirrhosis on the basis of clinical, biochemical and imaging methods and endoscopic signs. The severity of the disease was evaluated according to the Child-Pugh classification . Informed and written consent was obtained from each patient included and the study and was approved by institutional ethical committee.2.1. Exclusion Criteria
Patients with non alcoholic cirrhosis due to hepatitis B, hepatitis C, wilsons disease, haemochromatosis, α1 antitrypsin deficiency, toxic damage to the liver and having history of vitamin supplementation or antibiotic therapy were excluded. Blood samples were drawn from patients with LC on first day of admission.2.2. Markers of Liver Injury and Oxidative Stress
Serum total bilirubin, Serum aspartate transaminase (AST), albumin, and gamma glutamyl transferase (GGT) were measured in all of the patients. SOD, GPx, MDA levels were measured in 50 patients with cirrhosis and the results obtained were correlated with normal individual’s age matched and having no history of LC.2.3. SOD Measurement (U/gm/Hb)
The Quantitative in vitro determination of Superoxide Dismutase was performed by Randox Laboratories method. Erythrocytes were obtained for the superoxide dismutase activity which was measured using xanthine and xanthine oxidase (XOD) to generate superoxide radicals which react with 2- (4-iodophenyl)-3-(4-nitrophenol)-5-phenyltetrazolium chloride (I.N.T) to form a red formazan dye. The superoxide activity is then measured by the degree of inhibition of this reaction. One unit of SOD is that which causes 50% inhibition of the rate of reduction of INT under the conditions of the assay. The absorbance was measured at 505 nm .2.4. Glutathione Measurement (U/gm/Hb)
The Quantitative in vitro determination of glutathione Peroxidase (GPx) was performed from Randox Kit. The assay method utilizes glutathione oxidation by cumine hydroxide. The oxidation of NADPH to NADP+ was measured by the decrease in absorbance at 340 nm .2.5 Lipid Per-oxidation Measurement (MDA (nmol/ml))
The MDA was estimated by the standard method where the Serum lipid peroxide was mixed with Methyl Phenyl indole in acidified medium and after incubation for 48˚C for 60 minutes, the resultant chromophore intensity was recorded at 586 nm .2.6. Ferritin Estimation
Ferritin was estimated on the VIDAS (VITEK Immuno Diagnostic Assay System) instruments. VIDAS Ferritin is an automated quantitative test for use on the VIDAS instruments for the determination of human Ferritin in human serum using enzyme linked fluorescent assay (ELFA) technique. The assay combines a one-step immunoassay sandwich method with a final fluorescent detection. During the detection step, the substrate (4-Methyl umbelliferyl phosphate) is cycled in and out of the Solid Phase Receptacle (SPR). The conjugate enzyme catalyses the hydrolysis of this substrate in to a florescent product (4-Methyl umbelliferone) and the resulting florescence is measured at 450 nm. The intensity of the florescent is proportional to the concentration of antigens present in the sample [22, 23, 24].2.7. Statistical Analysis
The continuous variables were recorded as a Mean ± standard deviation. Statistical analysis was performed with open-epi software. The significance of difference between the various groups was calculated using the Correlation, ANOVA-f test to ascertain whether the differences between the various groups were clinically significant (P < 0.001).
The differences between the two groups of alcoholic cirrhotic patients and control subjects in terms of liver injury (GGT, AST, albumin and T.bilurubin) were statistically significant. All cirrhotic patients showed significant decrease in SOD (p < 0.0001) and GPx (p < 0.0001)and corresponding increase in MDA levels (p < 0.001) and ferritin levels (p<0.0001) when compared with control subjects as shown in Table 1. Serum AST levels and total bilirubin levels were found to be higher in cirrhotic patients with ALC2 than those with ALC1. Serum albumin levels were found to be lower in cirrhotic patients with ALC2 than those with ALC1. Serum GTT levels were found to be lower in patients with ALC2 than in ALC1 as shown in Table 2. Correlation coefficient (PEARSON’S) between the oxidative stress markers with indicators of liver injury in different alcohol consumption groups (r = -0.072 for ALC1 and r = -0.126 for ALC2) in liver cirrhosis was weakly significant.
The study results have indicated that there was accumulation of oxidative stress markers with increasing alcohol intake resulting in further damage to liver as revealed by abnormal liver function tests. This study results were similar to previous research reports which suggested that there was an increased risk of liver injury with chronic alcohol intake [25, 26]. Studies in the past have also revealed that there was an increase in iron load with chronic ethanol exposure [16, 27]. It has been experimentally demonstrated in chronic ethanol fed rats that ethanol tends to increase lipid peroxidation with iron acting as a cofactor in catalyzing lipid peroxidation and is responsible for elevated malondialdehyde (MDA) production .
Our study revealed that there was an increase in ferritin levels and subsequent rise in MDA levels associated with high alcohol consumption as observed by a previous study . A study by Clot P et al, which assessed the role of alcohol in oxidative damage of liver, revealed that decreased activities of glutathione were observed among cirrhotic patients (alcohol consuming group) with worsening Child Pugh score when compared with control subjects confirming the fact that there was stimulation of lipid peroxidation in patients with liver cirrhosis which is associated with alcohol abuse .
Previous studies have demonstrated that there was an association between reduced antioxidant levels and increased oxidative stress markers with increasing alcoholic consumption resulting in liver injury [29, 30, 31].
The current study results have also indicated that there was an added risk of liver injury with increased alcohol consumption as evident from the abnormal liver function tests. There was as a negative correlation of oxidative stress markers with levels of alcohol consumption and markers of liver injury.
Drawback of this study is that we could not evaluate extracellular MnSOD and cytosolic CuZnSOD activities which are released from damaged cells and are indicators of cell death.
In conclusion, it is evident from the results of this study and the existing literature that there was a compromise of antioxidant defense system with rise in oxidative stress markers and subsequent decrease in antioxidants levels between the two groups with alcohol consumption when compared to normal controls. The level of oxidative stress is indicated with elevated MDA levels and corresponding reduction in SOD and GPx levels which revealed significantly increased risk of liver injury related to excessive alcohol intake when compared with control group. Our results reemphasize the fact that with diminishing antioxidant levels and the rising oxidative stress with iron as central cofactor in producing injury, alcohol consumption and iron levels play a key role in the progression of liver disease.
Regular monitoring of oxidative stress markers and iron overload indicators in alcoholic patients is necessary for better patient management and to minimize the morbidity and mortality related to liver injury.
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