Figure 2. Histopathology of Myxobolus fomenai-infected skeletal muscle. A: Focal replacement of the skeletal muscle fibres with Myxobolus fomenai species plasmodium containing spores and developmental stages with moderate inflammatory reaction caused by plasmodium rupture, accompanied with hyaline degeneration of the surrounding muscle fibres, myomalacia, and intermuscular oedema. B: High power of A to show Myxobolus fomenai species spores and developmental stages. C: Several spores were seen disintegrating the adjacent muscle fibres causing their lysis. Leukocytes are seen in the vicinity of the damaged muscle fibres. D: Ruptured plasmodium of Myxobolus fomenai species plasmodium containing spores and developmental stages, surrounded with leukocytes. E-F: Morphometric measurement of a spore (E) and its polar capsules (F), their length and width. Polar capsules were equal, pear-shaped and occupied about two-thirds of the spore. G: Hyaline degeneration of some muscle fibres and aggregation of intermuscular erythrocytes and leukocytes (oval shape). H: Several leukocytes are seen disintegrating a muscle fibre. I: Partially obliterated blood vessel, located in the skeletal muscle fibres, with erythrocytes and hyperplastic and vacuolar changes in its wall. The blood vessel is surrounded with several erythrocytes (haemorrhage) and brownish deposits of possible haemosiderin pigment (byproduct of erythrocyte lysis). There is also oedema recorded around the affected blood vessel. J: A micrograph of an intermuscular blood vessel showing diapedesis (liberation of eryhrocytes) from the damaged blood vessel endothelium (oval shapes). K: An intermuscular blood vessel showing congestion, accumulation of intravascular mononuclear cells and erythrocytes, and vascular wall damage. Perivascular oedema is noticed. L: An intermuscular accumulation of extravasated erythrocytes and suspected haemosiderin deposition (brownish pigment). M: An intermuscular accumulation of mononuclear cells and erythrocytes. (10X: A, scale bar = 50 μm. 40X: B; C; D; E; F; G; H; I; J; K, scale bar = 10 μm. 1= Muscle; 2= Myxobolus spores; 3= oedema; 4= erythrocytes; 5= leukocytes.)


Implementation of Tissue Histopathology and Parasitic Morphometric Analysis in the Diagnosis of Myxobolus Fomenai Infection in the Skeletal Muscles Nile Tilapia

Hebatallah Ahmed Mahgoub, Ahmed Elnaggar, Jean-Rémy Sadeyen

American Journal of Infectious Diseases and Microbiology. 2017, 5(4), 137-142 doi:10.12691/ajidm-5-4-3