Figure 1. The effects of hydrogenized water on glutamate toxicity as monitored by DNA damage response (γH2AX). Human SH-SY5Y neuroblastoma cells were treated with a series of culture media containing various concentrations of hydrogenized water for 24 hr prior to exposure to glutamate. The protective effect of hydrogen against glutamate toxicity was determined by reductions in γH2AX as described in Materials and Methods. In this plot of the relative amounts of γH2AX (compared to an untreated control) versus hydrogen concentration the EC50 was calculated to be 0.030 mM H2

From

Hydrogenized Water Effects on Protection of Brain Cells from Oxidative Stress and Glutamate Toxicity

Robert Settineri, Jie Zhou, Jin Ji, Rita R. Ellithorpe, Steven Rosenblatt, Antonio Jimenez, Shigeo Ohta, Gonzalo Ferreira, Garth L. Nicolson

American Journal of Food and Nutrition. 2018, 6(1), 9-13 doi:10.12691/ajfn-6-1-2