Microbiological Assessment and Prevalence of Food Borne Pathogens in Street Vended Wara - Nig...

Omemu A.M, Obadina O. A, Taiwo G.J, Obuotor T.M.

  Open Access OPEN ACCESS  Peer Reviewed PEER-REVIEWED

Microbiological Assessment and Prevalence of Food Borne Pathogens in Street Vended Wara - Nigerian White Cheese

Omemu A.M1,, Obadina O. A2, Taiwo G.J3, Obuotor T.M.3

1Department of Hospitality and Tourism, Federal University of Agriculture, Abeokuta (FUNAAB)

2Department of Food Science and Technology, FUNAAB

3Department of Microbiology, FUNAAB

Abstract

Fifty white cheese (wara) samples were collected from ten local cheese hawkwer/vendors within Abeokuta, Nigeria to determine the microbial characteristics and the prevalence of some common food borne pathogen. The total aerobic plate counts (TPC) ranged from 6.8 to 9.5 log cfu/g; Lactic acid bacteria counts ranged from 2.8 to 6.9 log cfu/g while yeasts and mould counts ranged from 2.8 to 6.6 log cfu/g. Significant (p<0.05) variations were found between vendors and microbial population. The mean S. aureus count was 6.5 log cfu/g Salmonella, Presumptive E. coli and Bacillus cereus counts were 0.3, 2.4 and 3.3 log cfu/g respectively. B. cereus was found in 78%, Staphylococcus aureus in 100%, presumptive E. coli in 56% and Salmonella spp in 6% of the 50 wara cheese samples tested. None of the samples analyzed contained Vibrio cholerae. The results indicate that wara cheese presents a potential hazard for public health; and necessary precaution will have to be taken to improve the sanitary practices and cheese manufacturing technique.

At a glance: Figures

Cite this article:

  • A.M, Omemu, et al. "Microbiological Assessment and Prevalence of Food Borne Pathogens in Street Vended Wara - Nigerian White Cheese." American Journal of Food and Nutrition 2.4 (2014): 59-62.
  • A.M, O. , A, O. O. , G.J, T. , & T.M., O. (2014). Microbiological Assessment and Prevalence of Food Borne Pathogens in Street Vended Wara - Nigerian White Cheese. American Journal of Food and Nutrition, 2(4), 59-62.
  • A.M, Omemu, Obadina O. A, Taiwo G.J, and Obuotor T.M.. "Microbiological Assessment and Prevalence of Food Borne Pathogens in Street Vended Wara - Nigerian White Cheese." American Journal of Food and Nutrition 2, no. 4 (2014): 59-62.

Import into BibTeX Import into EndNote Import into RefMan Import into RefWorks

1. Introduction

Wara is a Nigerian soft, white, unsalted and unripened cheese usually processed from cow milk by the Fulani tribes who are mainly cattle rearers in Nigeria. Wara making is thought to have started with the Fulanis but as a result of their normadic lifestyle, it has spread to other parts including Kwara, Oyo, Ogun, Ondo and the Benin republic (Raheem, 2006). Wara is made from unpasteurized, unfermented whole cow milk by coagulation of the milk with the juice extract of Calotropis procera. Wara cheese is an excellent source of protein, fat and minerals such as calcium, iron and phosphorus, vitamins and essential amino acids and therefore is an important food in the diet of both young and old people. It also provides an ideal vehicle for preserving the valuable nutrients in milk and making them available throughout the year (O’Connor, 1993).

Presently, the wara hawked on the street in Abeokuta, Nigeria is produced at dairy farms on a small scale using raw milk from cows and traditional techniques passed down from generation to generation (O’Connor, 1993; Raheem, 2006).

Microorganisms are widely distributed in the products of animal origin. All foods possess a finite risk of microbiological contamination. The highest risk factors include foods of animal origin and foods consumed without prior cooking (Roberts, 1990). Wara cheese is one of those kinds of products. Microbial contamination of cheese may originate from various sources during cheese production. At the same time, pathogenic organisms may also be transferred to food by food handlers either directly or by cross-contamination (Temelli et al., 2006; Brito et al., 2008).

There have been outbreaks of infection associated with the consumption of cheese, and the predominant organisms responsible have included Salmonella, Listeria monocytogenes, verocytotoxin producing Escherichia coli (VTEC), and Staphylococcus aureus (De Buyser et al., 2001; Callon et al., 2008). Several outbreaks of food-borne illnesses associated with milk and dairy products indisputably revealed that cheeses, particularly made from unpasteurized or improperly pasteurized milk are main sources of Salmonellosis, Staphylococcal food poisoning and possibly E. coli infections (Doyle, 1991; IFST, 1998; Meng and Doyle, 1998).

Detailed investigations have demonstrated that the sources of contamination in cheese were raw milk, inadequately pasteurized milk, or post-pasteurization contamination with organisms originally derived from raw milk or from manufacturing environments.

The microbiological quality of wara cheese hawked on the street is of interest from a public health point of view since it is produced from raw milk, usually in small farm where basic facilities are absent, under poor hygienic practices, marketed by vendors who lack basic education on food safety and consumed raw without further processing. In addition, several articles have implicated street vended ready to eat food as positive vectors of food borne illnesses (Mosupye and von Holy, 1999; Edema and Omemu, 2004; Omemu, et al., 2005; Omemu and Aderoju, 2008)

The present study was undertaken to investigate the safety and quality of traditional ready-to-eat wara vended on the street in Abeokuta, Nigeria.

2. Materials and Methods

2.1. Sampling Procedure

Fifty ready-to-eat wara samples were randomly collected from 10 typical street vendors at a taxi park, university campus and a market within central Abeokuta. Three independent replicate surveys of each vendor were conducted.

Approximately 250 g of each sample was collected using the vendors serving utensils, placed into sterile container, transported on ice to the laboratory and analyzed upon arrival.

2.2. Microbiological Analysis

Ten gram (10 g) of each wara cheese sample was homogenized with 90 mL of 0.1% w/v buffered peptone water (BPW; Merck, Germany) in a stomacher (Laboratory Blender, Seward, UK) for 60 s at room temperature. Serial decimal dilutions in 0.1% BPW were made and duplicate 1 mL or 0.1 mL samples of appropriate dilutions were poured or spread on agar plates.

Total aerobic plate counts (TPC) were enumerated on Plate Count Agar (Difco, USA) using the pour-plate method and incubated at 30 ± 1°C for 72 h. Lactic acid bacteria were enumerated on De Man–Rogosa–Sharpe (MRS) agar (Oxoid, UK) incubated at 30 ± 1°C for 72 h, following the pour-plate method. Coliform group bacteria were enumerated on Violet Red Bile Dextrose agar (Difco,USA). The plates were overlaid prior to incubation at 37 ± 1°C for 24 h. Yeast and mould counts were enumerated on Saboraud Dextrose Agar (SDA) incubated at 25 ± 1°C for 5 days.

Incidence of food-borne pathogens: For the enumeration of B. cereus, 1 ml of the 10-1 dilution of each sample was plated onto Polymyxin-Pyruvate-Egg Yolk-Mannitol-Bromothymol blue Agar (PEMBA) plates (Oxoid) which were air-dried and incubated at 37°C for 24 to 48 hours. For the enrichment procedure, 10 ml of the 10-1 dilution was added to 90 ml Cooked Meat Medium (Oxoid) and incubated at 37°C for 48 hours (ICMSF, 1978). A loopful of the culture was then streaked onto PEMBA plates which were incubated 37°C for 48 hours. Peacock blue colonies with blue zones were subjected to appropriate biochemical tests (ISO, 1987).

Presumptive E. coli were enumerated in Trypton Bile X-glucuronide (TBX) medium (Oxoid CM 945). Incubation was at 30°C for 4 h then 18 h at 44°C. The number of blue/green colonies on the plates were then evaluated (Bridson, 1998).

S. aureus counts was done on Baird–Parker’s medium (Oxoid CM 275). Plates were surface inoculated and incubated at 37°C for 24-48h. The typical S. aureus colonies (black, convex, shiny with narrow white entire margin surrounded by clear zone) were counted and tested for coagulase reaction (Oxoid Staphytect Plus DR 850 M) (Bridson, 1998).

For the detection of Salmonella spp., 10 g of the sample was mixed with 90 ml Buffered Peptone Water (Oxoid), homogenized for two minutes and incubated at 37°C for 16 to 20 hours. The pre-enrichment broth culture (10ml) was added to 90ml of Tetrathionate Brilliant Green Broth (Oxoid) and 90ml of Selenite Cystein Broth (Oxoid) which were then incubated for 24 to 48 hours at 43°C and 37°C, respectively. A loopful of each enrichment broth culture was streaked onto Brilliant Green Agar (Oxoid) and Xylose-Lysine-Desoxycholate Agar plates (Oxoid). The plates were then incubated at 37°C for 24 to 48 hours (Arvanitidou et al., 1998). Appropriate biochemical tests were carried out on suspect colonies (ISO, 1993).

To determine the presence of Vibrio spp., 10g of each sample was mixed with Alkaline Peptone Water (90ml) (Oxoid), homogenised for two minutes and incubated at 37°C for 24 hours. A loopful of each enrichment culture was streaked onto a Thiosulphate Citrate Bile Salts Sucrose Agar plate (Oxoid) which was incubated at 37°C for 24 to 48 hours (ICMSF, 1996). Suspect colonies were subjected to appropriate biochemical tests.

2.3. Statistical Analysis

The count of colony-forming units (CFU) was transformed to logarithms (log CFU/g) prior to statistical treatment. The statistical analysis of the results was done by ANOVA using SPSS 11. The least significant difference (P < 0.05) of data is reported.

3. Results

Microbial Contents of wara cheese Samples: The microbial contents of the sampled cheese are presented in Table I. No significant differences (p>0.05) were observed between the 3 independent surveys for corresponding count types obtained from each vendor.

The average total bacterial counts (TPC) of the cheese samples ranged from 6.8 for vendor B to 9.5 log CFU/g for vendor I. Analysis of variance tests (ANOVA) indicated significant statistical differences (P<0.05) in the mean values of TPC obtained from the food vendors. Least significance difference (LSD) test used to compare the means indicated that the mean TPC observed for vendor B was significantly different (P<0.05) from mean TPC obtained for vendors H, I and J.

The lowest LAB count was 2.8 log CFU/g while the highest was 6.9 log CFU/g. Significant (p<0.05) differences were found in counts from different cheese vendors. Coliform counts of cheese samples ranged between 3.2 MPN/ml for vendor B to 4.8 log MPN/ml for vendor I. The yeasts and mould counts ranged from 2.8 log CFU/g for vendor B to 6.6 log CFU/g for Vendor I.

The highest TPC, LAB count, yeasts and mould counts were observed for vendor I while the least was observed with vendor B. High positive correlation (99% confidence level) was observed between microbial counts and different cheese vendors.

The mean counts of some food borne pathogens in wara cheese samples are presented in Table II. Bacillus cereus mean count was 3.3 log CFU/g with a range of 1.9 to 5.7 log CFU/g. Staphylococcus aureus ranged from 3.5-7.4 log CFU/g with a mean of 6.5 log CFU/g. The Salmonella counts ranged from 0.0 to 0.7 log CFU/g. No Vibrio cholerae was observed in any of the 50 cheese samples analyzed.

Figure 1 summarized the prevalence and frequency of occurrence of some food borne pathogens in the samples analyzed. The frequency of occurrence presented showed that B. cereus was found in 78%, Staphylococcus aureus in 100%, presumptive E. coli in 56% and Salmonella spp in 6% of the 50 wara cheese samples tested. None of the samples analyzed contained Vibrio cholerae.

4. Discussion

The high TPC observed in some wara samples in this study agrees with the findings of Warsama et al., (2006). The high total bacterial count in this study might be attributed to the use of low quality milk. According to Raheem (2006), the microbial quality of raw milk is crucial for the production of any high quality dairy food. It could also be as result of unsanitary conditions by the producers and vendors during processing and handling of the cheese. Mishandling and disregard of hygienic measures on the part of the food vendors may enable pathogens to come into contact with foods and in some cases to survive and multiply in sufficient numbers to cause illness in the consumer (Omemu and Aderoju, 2008).

The high coliform counts in the cheese samples were probably due to production of milk and cheese under poor conditions. According to international standards (Cyelan et al., 2003, Warsama, 2006), white cheese should not contain more than 100 CFU/ml coliforms bacteria (Law, 1999)

The high counts of S. aureus observed in this study is similar to the findings of Tekinsen and Ozdemir (2006), who found S. aureus counts ranging from 2.48–7.15 in 50 samples of Turkish Van otlu (Herb) cheese analyzed. This may be due partly to the use of raw milk and manufacturing process carried out under unhygienic conditions.

The occurrence of more than 102/g S. aureus in cheese samples may also indicate the use of milk from infected (clinical and subclinical mastitic) udder, which is frequently associated with this organism and/or an extensive contamination by personnel possibly involved in milk production and cheese making as human beings are common carriers (Hobbs and Gilbert, 1979).

Lactic acid bacteria are essential for fermentation and are acceptable in very large numbers mainly in natural cheese (Dolci et al., 2008). The presence of Salmonella sp in some of the cheese samples analyzed is contradictory to the results of Turantas et al. (1989) who did not find Salmonella spp. in 38 white cheese samples studied.

The occurrence of S. aureus and presumptive E. coli in more than 50% of the samples reveals an extensive deficiency of satisfactory sanitary practices during milk production and cheese manufacturing and/or post production handling of the wara cheese. S. aureus and E. coli in cheese are frequently used as indicators of hygienic quality and show lack of microbiological safety (IFST, 1998). Some strains of these organisms are known to cause diseases in humans. Therefore they are undesirable in high numbers.

In conclusion, due to the presence of some pathogenic bacteria in wara cheese sampled, there is a need to develop the production technique through adequate heat treatment, application of Good Manufacturing Practices (GMP) during processing and also improvements in hygienic practices during and after processing.

Table 1. Microbiological contents of wara cheese samples vended within Abeokuta metropolis in Nigeria

Table 2. Mean counts of food borne pathogens in samples of wara cheese vended within Abeokuta metropolis in Ogun state

Figure 1. Prevalence and frequency of occurrence of food borne pathogens in samples of wara cheese vended within Abeokuta metropolis in Ogun state

References

[1]  Arvanitidou, M., A. Tsakris, D. Sofianou and Katsouyannopoulos V. (1998). Antimicrobial resistance and R-factor transfer of salmonellae isolated from chicken carcasses in Greek hospitals. International Journal of Food Microbiology 40: 187-201.
In article      CrossRef
 
[2]  Bridson, E. Y. (1998).The oxoid manual (8th ed.). Basingstoke: Oxoid Ltd.
In article      
 
[3]  Brito, J. R. F., Santos, E. M. P., Arcuri, E. F., Lange, C. C., Brito, M. A. V. P., Souza, G. N. (2008). Retail survey of Brazilian milk and Minas frescal cheese and a contaminated dairy plant to establish prevalence, relatedness, and sources of Listeria monocytogenes isolates. Applied and Environmental Microbiology, 74, 4954-4961.
In article      CrossRef
 
[4]  Callon, C., Gilbert, F. B., Cremoux, R. D., and Montel, M. C. (2008). Application of variable number of tandem repeat analysis to determine the origin of S. aureus contamination from milk to cheese in goat cheese farms. Food Control, 19, 143-150.
In article      CrossRef
 
[5]  Ceylan, Z.G., Turkoglu, H. and Dayisoylu, S (2003). The microbiological and chemical quality of Skima cheese produced in Turkey. Pakistan Journal of Nutrition 2 (2): 95-97.
In article      CrossRef
 
[6]  De Buyser, M. L., Janin, F., and Dilasser, F. (1985). Contamination of ewe cheese with Staphylococcus aureus: Study of an outbreak of food poisoning. In J. Jeljaszewicz (Ed.), The Staphylococci (pp. 677-678). Stuttgart, Germany: Gustav Fisher.
In article      
 
[7]  Verlag. Dolci, P., Alessandria, V., Zeppa, G., Rantsiou, K. and Cocolin L. (2008). Microbiological characterization of artisanal Raschera PDO cheese: Analysis of its indigenous lactic acid bacteria. Food Microbiology 25: 392-399.
In article      CrossRef
 
[8]  Doyle, M. P. (1991). Escherichia coli O157: H7 and its significance in foods. International Journal of Food Microbiology, 12, 289-301.
In article      CrossRef
 
[9]  Edema, M. O. and Omemu, A.M. (2004). Microbiology and food hygiene in public food services. In: Proceedings of the international conference on science and national development (pp. 25-29).
In article      
 
[10]  Hobbs, B. C., and Gilbert, R.J. (1979). Food poisoning and food hygiene (4th ed.). London: Edward Arnold Ltd.
In article      
 
[11]  ICMSF (1996). Microorganisms in Foods 5-Microbiological specifications of food pathogens. Blackie Academic & Professional, London.
In article      
 
[12]  IFST Food safety and cheese (1998). Food Science and Technology Today, 12 (2), 117-122.
In article      
 
[13]  International Standards Organisation. (1993). Microbiology-General guidance on methods for the detection of Salmonella. ISO 6579. 3rd ed, International Standards Organisation, Geneva, Switzerland.
In article      
 
[14]  Law, B.A. (1999). Microbiological surveillance and control in cheese manufacture. In: Technology of Cheese Making (Law, B.A. ed.) Ed. Academic st press, pp: 251-280.
In article      
 
[15]  Mosupye, F. M., and von Holy, A. (1999). Microbiological quality and safety of ready-to-eat street-vended foods in Johannesburg, South Africa. Journal of Food Protection, 62, 1278-1284.
In article      
 
[16]  Meng, J. and Doyle, M. P. (1998). Microbiology of Shiga toxin producing Escherichia coli O157: H7 in foods. In J. B. Kaper and A. D. O_Brien (Eds.), Escherichia coli O157: H7 and other Shiga Toxin producing E. coli strains (pp. 92-108). Washington, DC: ASM.
In article      
 
[17]  O’Connor, C. B. (1993). Traditional cheese making manual. ILCA (International Livestock Centre for Africa), Addis Ababa, Ethiopia. Pp 56.
In article      
 
[18]  Omemu, A. M., Edema, M. O. and Bankole, M.O. (2005). Bacteriological assessment of street vended ready to eat (RTE) vegetables and prepacked salad in Nigeria. Nigerian Journal of Microbiology, 19 (1-2), 497-504.
In article      
 
[19]  Omemu, A.M. and Aderoju, S.T. (2008). Food safety knowledge and practices of street food vendors in the city of Abeokuta, Nigeria. Food Control, 19 396-402.
In article      CrossRef
 
[20]  Raheem, D. (2006). Developments and Microbiological Applications in African Foods: Emphasis on Nigerian Wara Cheese. Academic dissertation, University of Helsinki, Finland. Pp 45.
In article      
 
[21]  Roberts D.(1990) Food-borne illnesses. Source of infection: food. The Lancet 336 859-861.
In article      CrossRef
 
[22]  Tekinsen, K., and Ozdemir, Z. (2006). Prevalence of Microbiological and compositional status of Turkish foodborne pathogens in Turkish Van otlu (Herb) white cheese. Food Control, 17: 707-711.
In article      CrossRef
 
[23]  Temelli, S., Anar, S., Sen, C., and Akyuva, P. (2006). Determination of microbiological contamination sources during Turkish white cheese production. Food Control, 17, 856-861.
In article      CrossRef
 
[24]  Turantas F. Unluturk, A. and Goktan, D. (1989). Microbiological and compositional status of Turkish white cheese. International Journal of Food Microbiology, 8 (1), 19-24.
In article      CrossRef
 
[25]  Warsama, L.M., Ibtisam, E.M. Zubeir, E. l and Owni, O.A.O.(2006). Composition and hygienic quality of Sudanese soft cheese in Khartoum North (Sudan). International Journal of Dairy Science, 1 (1): 36-43. 2006.
In article      
 
  • CiteULikeCiteULike
  • MendeleyMendeley
  • StumbleUponStumbleUpon
  • Add to DeliciousDelicious
  • FacebookFacebook
  • TwitterTwitter
  • LinkedInLinkedIn