Figure 4. LC3B protein expression in PBTC evaluated by western blot after 72 hr cultivation. (+) - cells were treated with 10-4M Dex under normal culture conditions; (-) - cultivation without Dex. 1 - Protein marker. 2, 3 - cell lysates from healthy donor. Results confirm that nutrient depletion don't induces autophagy and suggest that Dex treatment for 72 hr may not induce autophagy in healthy donors. 4,5 - cell lysates from patients with mild asthma. Immunoblots results indicating that prolonged culturing and treatment of T-cells with Dex did not affect autophagy. 6,7 - cell lysates from patients with severe asthma. Immunoblots data indicating that prolong culturing and 72 hr treatment of T-cells with Dex induces autophagy : Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma : Science and Education Publishing

Figure 4. LC3B protein expression in PBTC evaluated by western blot after 72 hr cultivation. (+) - cells were treated with 10^-4M Dex under normal culture conditions; (-) - cultivation without Dex. 1 - Protein marker. 2, 3 - cell lysates from healthy donor. Results confirm that nutrient depletion don't induces autophagy and suggest that Dex treatment for 72 hr may not induce autophagy in healthy donors. 4,5 - cell lysates from patients with mild asthma. Immunoblots results indicating that prolonged culturing and treatment of T-cells with Dex did not affect autophagy. 6,7 - cell lysates from patients with severe asthma. Immunoblots data indicating that prolong culturing and 72 hr treatment of T-cells with Dex induces autophagy

American Journal of Biomedical Research. 2021, 9(1), 10-18 doi:10.12691/ajbr-9-1-3