Figure 2. Smad protein expression in TGF-β1-stimulated NRCMs. NRCMs were treated with TGF-β1 (3 μg/L) for 15 min, 30 min, 1 h, 2 h, or 24 h. (A) The protein expressions of Smad2, phospho-Smad2 (pSmad2; Ser465/467), and total Smad2/3 were determined using Western blot analysis. (B) Quantification of (A). β-actin was used as an internal control. Data are expressed as mean ± SD. *P < 0.05 vs. control (n=7)


Puerarin Attenuates Transforming Growth Factor Beta-1 Induced Hypertrophic Responses and Smad Proetin Upregulation in Neonatal Rat Cardiomyocytes

Yongying Shi, Aijun Liu, Junjiang Lu, Guangyuan Chen, Shiming Liu, Minsheng Chen, Chengfeng Luo

American Journal of Biomedical Research. 2020, 8(2), 40-46 doi:10.12691/ajbr-8-2-3