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Influence of Organic Acids on Kinetic Properties of Engineered Phlebia radiata MnP3 Enzymes (Wild-type and Mutants) during Mn (II) Oxidation

Usenobong F. Ufot, Imeh J. Okop, Dorcas U. Ufot, Aniefiok E. Ite, Monday I. Akpanabiatu

American Journal of Biomedical Research. 2022, 10(1), 9-13 doi:10.12691/ajbr-10-1-2
  • Table 1. Mn (II) oxidation by wild-type and mutant rPr-MnP3 with different organic acid Mn (III) chelators. As described in section 2.0. Oxidations of Mn (II) was carried out using 100 mM of appropriate buffer (sodium tartrate, sodium malonate or sodium lactate) pH 5.0. Reaction mixtures contained 0.03 – 0.2µM MnP3, 0.1mM H2O2 and 0.2 -1.2 mM MnSO4 for wild-type and 1 – 45 mM MnSO4 for mutant enzymes. Results are the average of three determinations with the standard errors indicated