Open Access Peer-reviewed

Evaluation of Acetaminophen Effect on Oxidative Stressed Mice by Peroxide Hydrogen

BENKHASSI Zoubair1, LAHLOU Fatima Azzahra1,, HMIMID Fouzia1, LOUTFI Mohammed1, BENAJI Brahim2, BOURHIM Noureddine1

1Biochemistry and Molecular Biology laboratory, Ain Chock Faculty of Science Hassan II University, Casablanca, Morocco

2Microbiology pharmacology Biotechnology and Environment laboratory, Ain Chock Faculty of Science Hassan II University, Casablanca, Morocco

American Journal of Biomedical Research. 2013, 1(4), 75-79. DOI: 10.12691/ajbr-1-4-2
Published online: August 25, 2017

Abstract

Acetaminophen (Paracetamol) is among the most commonly used analgesic and antipyretic drugs worldwide, it’s often, but anomalously, classified as non-steroidal anti-inflammatory drugs (NSAIDs) in textbooks of pharmacology . This study aims to evaluate if paracetamol has an antioxidant effect, relative to its analgesic antipyretic and weak anti-inflammatory activities, or it possesses a cytotoxic potential. Oxidative stress was induced by intraperetoneal injection of peroxide hydrogen (H2O2), and then a comparative study is made concerning the activities of the antioxidant enzymes SOD, CAT, and GR as well as lipid peroxidation levels in liver. An increase in SOD, CAT, GR activity and lipid peroxidation in mice treated with H2O2 accompanied by paracetamol; compared to the group treated by vitamin C + H2O2 showed that acetaminophen doesn’t show any antioxidant effect. Moreover this study has suggested that acetaminophen induced cytotoxicity in liver mediated by increased oxidative stress and altered redox metabolism.

Keywords:

acetaminophen (paracetamol), peroxide hydrogen, oxidative stress
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